Towards a multimedia remote viewer for mobile thin clients

Be there a traditional mobile user wanting to connect to a remote multimedia server. In order to allow them to enjoy the same user experience remotely (play, interact, edit, store and share capabilities) as in a traditional fixed LAN environment, several dead-locks are to be dealt with: (1) a heavy and heterogeneous content should be sent through a bandwidth constrained network; (2) the displayed content should be of good quality; (3) user interaction should be processed in real-time and (4) the complexity of the practical solution should not exceed the features of the mobile client in terms of CPU, memory and battery. The present paper takes this challenge and presents a fully operational MPEG-4 BiFS solution

Thermal noise limit in the Virgo mirror suspension

Abstract The expected current limit to the Virgo sensitivity is presented. New materials to realize a low thermal noise suspension for the Virgo optics are investigated. A promising fused silica suspension for the Virgo mirrors is presented

Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regulon in response and tolerance of Saccharomyces cerevisiae to stress induced by sulfur dioxide

During vinification Saccharomyces cerevisiae cells are frequently exposed to high concentrations of sulfur dioxide (SO2) that is used to avoid overgrowth of unwanted bacteria or fungi present in the must. Up to now the characterization of the molecular mechanisms by which S. cerevisiae responds and tolerates SO2 was focused on the role of the sulfite efflux pump Ssu1 and investigation on the involvement of other players has been scarce, especially at a genome-wide level. In this work, we uncovered the essential role of the poorly characterized transcription factor Com2 in tolerance and response of S. cerevisiae to stress induced by SO2 at the enologically relevant pH of 3.5. Transcriptomic analysis revealed that Com2 controls, directly or indirectly, the expression of more than 80% of the genes activated by SO2, a percentage much higher than the one that could be attributed to any other stress-responsive transcription factor. Large-scale phenotyping of the yeast haploid mutant collection led to the identification of 50 Com2-targets contributing to the protection against SO2 including all the genes that compose the sulfate reduction pathway (MET3, MET14, MET16, MET5, MET10) and the majority of the genes required for biosynthesis of lysine (LYS2, LYS21, LYS20, LYS14, LYS4, LYS5, LYS1 and LYS9) or arginine (ARG5,6, ARG4, ARG2, ARG3, ARG7, ARG8, ORT1 and CPA1). Other uncovered determinants of resistance to SO2 (not under the control of Com2) included genes required for function and assembly of the vacuolar proton pump and enzymes of the antioxidant defense, consistent with the observed cytosolic and mitochondrial accumulation of reactive oxygen species in SO2-stressed yeast cells.This work was funded by INNOVINE&WINE, Norte-01-0145-FEDER-000038, co-financed by the European Region-al Development Fund (ERDF) through Norte 2020 and by ERFD through POCI-COMPETE 2020. Support received by FCT-Portuguese Foundationfor Science and Technology(PTDC/EXPL/AGR-TEC/1823/2013 and PTDC/AGR-TEC/3315/2014) and by INTERACT project –“Integrated Research in Environment, Agro-Chain and Technology”, no. NORTE-01-0145-FEDER-000017, in its line of research enti-tled VitalityWine. Supportreceived by Biosystems and In-tegrative Sciences Institute (BioISI; FCT/UID/Multi/04046/2018) and iBB-Institute for Bioengi-neering and Biosciences (UID/BIO/04565/2019) by FCT and from Programa Operacional Regional de Lisboa 2020 (pro-ject no. 007317 and PTDC/AGR-TEC/3315/2014_LISBOA-01-0145-FEDER-016834) is also acknowledged.The authors thank Professor Isabel Sá-Correia for the help and guidance in conducting the chemogenomic analysi

AC Electric Fields Drive Steady Flows in Flames

We show that time-oscillating electric fields applied to plasmas present in flames create steady flows of gas. Ions generated within the flame move in the field and migrate a distance δ before recombining; the net flow of ions away from the flame creates a time-averaged force that drives the steady flows observed experimentally. A quantitative model describes the response of the flame and reveals how δ decreases as the frequency of the applied field increases. Interestingly, above a critical frequency, ac fields can be used to manipulate flames at a distance without the need for proximal electrodes.Chemistry and Chemical BiologyEngineering and Applied Science

High-fidelity imaging in brain-wide structural studies using light-sheet microscopy

Light-sheet microscopy (LSM) has proven a useful tool in neuroscience to image whole brains with high frame rates at cellular resolution and, in combination with tissue clearing methods, is often employed to reconstruct the cyto-architecture over the intact mouse brain. Inherently to LSM, however, residual opaque objects, always present to some extent even in extremely well optically cleared samples, cause stripe artifacts, which, in the best case, severely affect image homogeneity and, in the worst case, completely obscure features of interest. Here, demonstrating two example applications in intact optically cleared mouse brains, we report how Bessel beams reduce streaking artifacts and produce high-fidelity structural data for the brain-wide morphology of neuronal and vascular networks. We found that a third of the imaged volume of the brain was affected by strong striated image intensity inhomogeneity and, furthermore, a significant amount of information content lost with Gaussian illumination was accessible when interrogated with Bessel beams. In conclusion, Bessel beams produce high-fidelity structural data of improved image homogeneity and might significantly relax demands placed on the automated tools to count, trace, or segment fluorescent features of interest

E-Defense 2015 ten-story building: beam–column joint assessment according to different code-based design

Recent devastating earthquakes worldwide pointed out the importance of seismic detailing and their influence on the observed damage and subsequent repairability of reinforced concrete buildings. Several studies and post-earthquake observations remarked the role of beam–column joints (BCJs) on the global building response and the effectiveness of transverse reinforcement in increasing the joint shear strength and the ultimate deformation. Although number of experimental and theoretical studies focused on the seismic response of BCJs, their mechanical behaviour is still a discussed topic. This resulted in number of design approaches available in worldwide code or standards that lead to different quantity of joint stirrups. This study focuses on the response of BCJs of a 10-story prototype building designed according to Japanese standards and tested in 2015 on the E-Defense shaking table. First the damage assessment at global (building) and local (joint) level is performed at increasing intensities and considering the building in the base slip and base fixed configurations. A refined numerical model is then developed and validated against global and local experimental results. Then, the joint stirrups are re-designed according to different international standards (ACI, EC8, NZS) and different numerical models are developed. The numerical results are then compared in terms of interstorey drift demand and joint shear strain. Finally, a comparison in terms of expected damage varying the design approach of joint stirrups is proposed

Nanofabrication by self-assembly

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